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Chuck Reynolds
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Chuck Reynolds   My Press Releases

The human effects of resveratrol. F-2

Published on 4/20/2019
For additional information  Click Here

Differential effects of resveratrol on androgen-responsive LNCaP human prostate cancer cells in vitro and in vivo. F-2

                     

Effects of resveratrol on ARG expression and affinity of resveratrol for AR.

(A) Microarray analysis of ARG gene expression. LNCaP cells cultured in 10% FBS were treated with 0, 1, 5 or 25 μM resveratrol for 48 h, and total RNA isolation and microarray analysis for ARG expression were performed as described in Materials and Methods. Triplicate treatments and analyses were conducted for each concentration. Results are expressed as a heat map. (Red color: upregulated gene and green color: downregulated gene.) Intensity of the color is proportional to the magnitude of effects on a log 2 scale, −3 to +3.

(B) RT–PCR analysis of effects of resveratrol on ARG expression. LNCaP cells cultured in 10% FBS were treated with or without resveratrol (25 μM) for 48 h, total RNA was isolated and mRNA levels of selected ARGs (B2M, PSA, SEPP1 and STK39) were determined as described in Materials and Methods. Results are expressed as mean ± SD (n = 3). Bar with * is significantly different from vehicle control at P < 0.05.

(C) RT–PCR analysis of effects of resveratrol on IGF-1R expression. LNCaP cells cultured in 10% FBS were treated with 0, 1, 5 or 25 μM resveratrol for 48 h, and total RNA was isolated and mRNA levels of IGF-1R determined as described in Materials and Methods. Results are expressed as mean ± SD (n = 3). Bars with *are significantly different from vehicle control (0) at P < 0.05.

(D) RT–PCR analysis of effects of resveratrol on CDKN1A expression. LNCaP cells cultured in 10% FBS were treated with 0, 1, 5 or 25 μM resveratrol for 48 h, and total RNA was isolated and mRNA levels of CDKN1A determined as described in Materials and Methods. Results are expressed as mean ± SD (n = 3). Bar with *is significantly different from vehicle control (0) at P < 0.05.

(E) Affinity of resveratrol for AR. AR binding assays were performed as described in Materials and Methods. Dihydrotestosterone (DHT) was used as positive control. Results are expressed as percent control ± SD (n = 3). Points with *are significantly different from vehicle control (0) at P < 0.05.

Article Produced By

Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agriculture Research Service, United States Department of Agriculture, 10300 Baltimore Avenue, Building 307C, Room 132, Beltsville, MD. Laboratory of Cellular Regulation and Carcinogenesis, National Cancer Institutes, National Institutes of Health, Department of Nutrition and Food Science, University of Maryland, College Park, Department of Pharmaceutical Sciences and Pharmacology and Toxicology Graduate Program College of Pharmacy, Washington State University, Pullman, National Food Research Institute, Tsukuba, Ibaraki 305-8642, Japan. Nutritional Sciences Research Group, Division of Cancer Prevention, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Biometrical Consulting Service, Beltsville Area, Agriculture Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA. Division of Nutritional Sciences, University of Texas at Austin, Austin, TX 78712, USA. Department of Carcinogenesis, MD Anderson Cancer Center, Smithville, TX 78957, USA
 

Posted by
Chuck Reynolds

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Chuck
559-369-7791
chuck@dreams-inn.com
https://chuck212.amazingrape.com/

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